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Title: Coregulation of biosynthetic genes and transcription factors for aporphine-Ttpe alkaloid production in wounded lotus provides insight into the biosynthetic pathway of nuciferine
Authors: Thitirat Meelaph
Khwanlada Kobtrakul
N. Nopchai Chansilpa
Han, Yuepeng
Rani, Dolly
Wanchai De-Eknamkul
Sornkanok Vimolmangkang
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Other author: Chulalongkorn University. Faculty of Pharmaceutical Science
Issue Date: 8-Aug-2018
Publisher: American Chemical Society
Citation: ACS Omega. 3,8 (2018) : p.8794−8802
Abstract: Lotus (Nelumbo nucifera Gaertn.) contains various bioactive compounds, with benzylisoquinoline alkaloids (BIAs) as one of the major groups. The biosynthetic pathways of two major bioactive BIAs in this plant, nuciferine and N-nornuciferine, are still not clear. Therefore, several genes related to BIA biosynthesis were searched from the lotus database to study the role of key genes in regulating these pathways. In this study, the expression profiles of NCS, CNMT, 6OMT, CYP80G2, and WRKY TFs were investigated in mechanically wounded lotus leaves. It was found that the accumulation of nuciferine and N-nornuciferine significantly increased in the mechanically wounded lotus leaves in accordance with the relative expression of putative CYP80G2 and one WRKY transcription factor (NNU_24385), with the coregulation of CNMT. Furthermore, the role of methyltransferase-related genes in this study suggested that methylation of the isoquinoline nucleus to yield a methylated-BIA structure may occur at the N position before the O position. Altogether, this study provides improved understanding of the genes regulating BIA biosynthesis under stressed conditions, which could lead to improvements in BIA production from the commercial lotus.
ISSN: 2470-1343
metadata.dc.identifier.DOI: 10.1021/acsomega.8b00827
Type: Article
Appears in Collections:Chula Scholars - 2018

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