Separation of isoflavones from soybean flake extracts by high performance liquid chromatography

Abstract

The simulation and the useful information for the separation of isoflavones (Daidzin and Genistin) in soybean flake extracts by High Performance Liquid Chromatography (HPLC) were obtained. In this study, the most suitable mobile phase for the separation of isoflavones from soybean flake extracts was 33% methanol aqueous solution. For setting up the simulation, the mass balance and linear adsorption isotherm were used. The modeling parameters in the simulation estimated from the experiment and the correlation were internal porosity, external porosity, axial dispersion coefficient, adsorption equilibrium constant, and mass transfer coefficient. It was found that the simulation without the axial dispersion term was the same as the simulation with this term but required time eight to nine-fold less. The simulation could predict the separation in HPLC system well when the injected concentration and the velocity of mobile phase were changed. Furthermore, the relationship between the adsorption equilibrium constant and the concentration of methanol in the mobile phase in the range of 25% to 40% vol/vol aqueous solution was found from the experiment to be linear