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Title: Effects of genistein supplementation on gingival blood flow disturbances after menopause: an in vivo study using ovariectomized rats
Authors: Supathra Amatyakul
Achareeya Kasiyaphat
Suthiluk Patumraj
Siriporn Chotipaibulpan
Prasong Siriviriyakul
Other author: Chulalongkorn University. Faculty of Dentistry
Srinakharinwirot University. Faculty of Physical Education
Chulalongkorn University. Faculty of Medicine
Chulalongkorn University. Faculty of Dentistry
Chulalongkorn University. Faculty of Medicine
Subjects: Genistein
Issue Date: 2007
Publisher: Chulalongkorn University
Citation: Asian biomedicine : research, reviews and news. 1,4(December 2007): 421-424
Abstract: Background: Estrogen synthesis decreases in the postmenopausal period inducing gingival periodontal disturbance. Estrogen replacement therapy has been used to reduce such gingival complications, although it has been limited by the adverse effect of estrogen. Objective: To investigate the effects of supplementation of genistein, a phytoestrogen derived from the soybean, on blood flow disturbance in the gingiva after menopause, using an ovariectomized rat model. Materials and methods: Female Wistar rats (220 - 280 g) were subjected to a bilateral ovariectomy (OVX rats). The rats were classified into four groups: sham-operated animals treated with vehicle (DMSO 100 μl/day,s.c) (Sham (veh); n = 6), OVX rats treated with vehicle (DMSO 100 μl/day,s.c) (OVX (veh); n = 6), OVX rats treated with 17β-estradiol (20 μg/kg/day,s.c) (OVX (E2); n = 6) and OVX treated with genistein (0.25 mg/kg.BW/day,s.c) (OVX (gen); n = 6). Plasma estradiol, body weight, and gingival blood flow (GBF) were measured 3 weeks after OVX. For the GBF measurement, laser Doppler perfusion flowmetry was used. Results: Three weeks after OVX, plasma estradiol levels in three groups of OVX rats were significantly lower than the sham-operated control (p < 0.05). Body weight increased in OVX (veh) and OVX (gen) groups (p < 0.001). The GBF increased significantly in OVX (veh) compared to the Sham (veh) group (p < 0.05), indicating that GBF disturbance due to the lack of estrogen was improved by oral supplemention of 17β-estradiol or genistein. Conclusion: Genistein supplementation improves disturbed gingival blood flow induced in ovariectomized-rats. Genistein might have oral health benefits after menopause.
ISSN: 1905-7415
Type: Article
Appears in Collections:Med - Journal Articles

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