Protective effects of glycine max seed extract and chrysanthemum indicum flower extract against cisplatin-induced renal cell death

Abstract

Although cisplatin has been accepted as one of the most efficient chemotherapeutica gentsf or the treatmento f solid tumors, frequently observed nephrotoxicityh as limited its use in severapl atients.T he purposeo f this studyw as to investigate the protective effect of Glycine max seed extract (GM) and Chrysanthemumin dicun flower extract (CM) on cisplatin-induceda poptosisi n human proximal tubular epithelial HK-2 cells. Cisplatin was shown to induce cellular reactive oxygen species (ROS) up-regulation playing a key role in HK-2 cell apoptosis.P retreatmenot f the cells with GM or CM and cisplatin resultedi n the inhibition of ROS inductiona nd a decreaseo f cell apoptosisC. isplatini nduced renal cell toxicity via its ability to increasec ellular hydrogenp eroxide and hydroxyl radical levels. GM protects cisplatin-induced apoptosis by the mechanism which involves its anti-oxidant activity against only intracellular hydrogenp eroxide,w hile CM could scavengeb oth of hydrogenp eroxidea nd' hydroxyl radical. Furthermore, our results demonstrated that GM and CM selectively protect renal cells without significant interfering effect on cisplatin toxicity in non-small cell lung cancer H460 and melanoma G36l cells. These findings indicate a novel activity for GM and CM extracts protection of cisplatininduced nephrotoxicity which could benefit the development for nephroprotective approaches.