Cytotoxicity against cancer cell lines of stem bark of croton oblongifolius Roxb. from Amphoe Muang, Sakolnakorn Province

Abstract

The chemical investigation of the stem bark of Croton oblongifolius Roxb. from Sakolnakorn province gave five compounds from hexane crude extract. The structure of these compounds were established on the basis of physical properties and spectroscopic data including IR, MS, 1D-NMR and 2D-NMR techniques. Two cembrane diterpenoids; crotocembraneic acid (1), and neocrotocembraneic acid (2), one halimane diterpenoid; crotohalimaneic acid (3), one clerodane diterpenoid; (-)-20-benzyloxyhardwickiic acid (4), and one sinapyl alcohol; 3-(3,5-dimethoxy-4-hydroxy phenyl) propanyl benzoate (5), were identified. All of the compounds were tested for cytotoxicity against human cancer cell lines. crotohalimaneic acid (3), and 3-(3,5-dimethoxy-4-hydroxy phenyl) propanyl benzoate (5) have high activity against 5 cancer cell lines; Hep-G2 (hepatoma), SW620 (colon), Chago (lung), Kato-3 (gastric) and BT474 (breast).