Contamination level determination of Bisphenol-A-Diglycidyl Ether, Bisphenol-F-Diglycidyl Ether and certain derivatives from can coatings in oil-based foods manufactured in Thailand

Abstract

This research described systematic HPLC method development for the separation of BADGE, BFDGE and their monomeric derivatives (BADGE.HC1, BADGE.2HC1 and BFDGE.2HC1) in oil-based canned foods. The analysis was carried out using RP-HPLC with fluorescence detector. Optimum chromatographic conditions are: C18 ODS Hypersil (4.0x150 mm, 5 µm), 60% methanol in water mobile phase flow rate 0.75 mL/min, fluorescence detector λex277/λem313 nm. Satisfactory validation parameters were obtained: linearity of detector response ranged 0.035 -5.000 µg/mL (R²> 0.9990); standard calibrations were constructed from 0.035 -1.000 1.1.g/mL (R²> 0.9990); limits of detection ranged 0.012-0.033 µg/mL: limits of quantitation ranged 0.041- 0.113 µg/mL. Method precision and percent recovery were acceptable with coefficient of variance (CV) at LoQ and 5*LoQ spiking levels less than 15 and 7 respectively. Percent recovery was achieved at 64-85%. The method was found to be robusted and no deviation was detected when minor changes in condition were applied. The method was used to test 20 oil-based foods, all were made in Thailand. All samples were contaminated. Twenty-five percent were contaminated above the EU control limit of 1 mg/kg. Seventy percent are contaminated more than 0.200 mg/kg. The maximum contamination was 4.298 mg/kg and the minimum was 0.079 mg/kg.