Comparison of antimicrobial resistance and DNA patterns of Salmonella Schwarzengrund isolated from human and chicken meat in Thailand

Abstract

Salmonella is a bacterial agents that can cause disease in human and animals so it is a public health and economic problem in Thailand. The epidemiological report of Salmonella Schwarzengrund from WHO National Salmonella and Shigella Center, the National Institute of Health, Department of Medical Science, Ministry of Public Health in the year 1999-2003 shown that the prevalent of Salmonella Schwarzengrund isolated from human was changed from 0.2% to 2.6% and the prevalent of Salmonella Schwarzengrund isolated from chicken meat was changed from 0.3% to 26%. So 300 isolates of Salmonella Schwarzengrund from infection in human and chicken meat were studied epidemiological typing by tested 12 antimicrobial drugs: Ampicillin, Chloramphenical, Ciprofloxacin, Gentamycin, Nalidixic acid, Neomycin, Streptomycin, Sulfamethoxazole, Sulfamethoxazole+Trimethoprim, Tetracycline, cefotaxime, and cefotaxime+clavalunic acid were utilized in the susceptibility test of Salmonella Schwarzengrund and all of these samples were compare DNA profile between Salmonella Schwarzengrund from infection in human and chicken meat in Thailand by using Pulse field gel electrophoresis (PFGE). This study concluded that Salmonella Schwarzengrund is a multidrug resistance and the range is increased every year. About PFGE patterns suggest that Salmonella Schwarzengrund isolated from human and chicken meat have had a high similarity more than 70% so it should be mentioned that the nature source of human Salmonella Schwarzengrund is a same clone with Salmonella Schwarzengrund isolated from chicken meat.The acknowledge from this research will lead to better understanding about epidemiology and transmission of Salmonella schwarzengrund isolated in Thailand.