Comparative Study of three remineralizing products on changing the Enamel white spot lesion in vitro

Abstract

Objective: To compare optical appearance of white spot lesion before and after intervention with three remineralizing products and determine which one has the highest potency by using computerized image analysis. Materials and methods: Ninety human tooth crowns were coated with an acid resistant varnish, leaving a small window of sound enamel parallel to the horizontal plane. Artificial white spot lesion was created by thirty minutes immersion in demineralizing solution. Samples were randomly allocated into 3 groups : group A (GC tooth mousse : Casein Phosphopeptide - Amorphous Calcium Phosphate), group B (Clinpro tooth crème : 0.21% w/w sodium fluoride anti-cavity paste with tri-calcium phosphate) and group C (Prevident : 1.1% w/v Sodium Fluoride) are subjected to 60 remin/demin cycles. Photographs of each sample before and after intervention were taken at fifteen degree angle to the enamel surface and analyzed using computerized image analysis. Data was measured in term of LI% (luminance intensity proportionality) for optical appearance measurement. Results: Paired t-test showed that LI% before and after intervention of three groups were significantly different (p<0.001) at 99% confidence level. One-way ANOVA and Bonferroni statistics showed that changed LI% comparing between group A/ group C and group B/ group C were significantly different (p<0.050) while group A/ group B was not significantly different (p=1.000) at 95% confidence level. Conclusion: Optical appearance of white spot lesion was decreased after applied three remineralizing products and group C (Prevident : 1.1% w/v Sodium Fluoride) represented the highest change on white spot lesion.