Carboxymethylcellulose production from celluloseic gel by Acetobacter xylinum

Abstract

This research has the objective to study the various factors affecting cellulosic gel production by A.xylinum and to study the optimum conditions of CMC production from cellulosic gel. Cellulosic gel forming ability of A.xylimum was depend upon A.xylimum strains. The ST strain could form the highest amount of cellulosic gel when compared with DK and AGR60 strains. The suitable kinds of sugar using as C-dource for A.xylimum were glucose, sucrose and fructose. It was found that the cellulosic gel formation of these three strains in coconut water medium provided the higher yield than in synthetic medium. The surface of medium influenced on the gel forming ability. The maximum cellulosic gel were obtained from the 168.75 and 337.5 cm2 of surface area of medium. The optimum conditions of CMC production were as followed : the ratio of nata cellulose powder : distilled water of 1.0:2.0 (w/v) and the ratio of nata cellulose powder : NaOH : CICH2COONa of 1.0:3.0:0.44. The reaction mixture was mixed for 6 hours and incubated at room temperature for 36 hours. The reaction mixture was dissolved in hot water by the ratio of 1.0:12.5 of the reaction mixture to hot water and precipitated by 70% final concentration of ethanol. The viscosity of 1% of CMC solution at 25℃ and DS of CMC obtained were 334 cP and 0.48, respectively.