The effect of flowable resin composites on cultured human periodontal ligament cells compared with MTA

Abstract

The objective of this study was to investigate effect of flowable resin composites (Tetric R Flow, Filte TM Flow และ Aeliteflo TM) compared with MTA on cultured human periodontal ligament cells. The effect of the elution from materials to human periodontal ligament cells after 1, 2, 3 and 4 days immersion of material in cultured medium and also the direct contact on these material surfaces were observed in this study. The study also focused on the morphological appearances of cells in contact with material surfaces using scanning electron microscope (SEM). The result showed that elution from materials was not cytotoxic at all periods. But in the beginning, Filtek TM Flow presented more cytotoxicity compared to control. However, after cells were in direct contact to materials, freshly mixed of all material had high cytotoxicity to human periodontal ligament cells. MTA showed to be significantly more cytotoxic than Tetric R Flow at this period (p<0.05). But cytotoxicity level of MTA decreased rapidly after 1 day of immersion and became not different from Tetric R Flow and Filtek TM Flow. Aeliteflo TM, on the other hand, revealed significantly more toxicity compared to others until 2 days after immersion. For morphological assay. Tetric R Flow and MTA demonstrated good spreading and adhesion of cultured HPDL cells Filtek TM Flow revealed good spreading of cells but not well attach to material. Aeliteflo TM was the most toxic showing cell in discoid shape and not attached well on the material surface. It was concluded that eventhough freshly mixed materials were toxic, after materials were immersed HPDL cells could normally grow and attach on flowable resin composites and MTA. But Tetric R Flow, in particular, had more positive effects on HPDL cells than other materials.