Nowadays, the magneto-and electro-active materials have attracted more attention to the researchers because of its wide variety of applications such as actuator, sensor, information storage and electromagnetic shielding. In the present study, the magnetically and electrically responsive bacterial cellulose was successfully prepared by step-wised synthesis of magnetic particles (Fe₃O₄) and silver particles (Ag) into bacterial cellulose matrix. The magnetic and silver particles were step-wised synthesized into bacterial cellulose matrix by using an ammonia gas-enhancing in situ co-precipitation method. Firstly, bacterial cellulse pellicle was immersed in an aqueous solution containing FeCl₃ and FeSo₄. After bacterial cellulose was treated with ammonia gas, the absorbed Fe²⁺ and Fe³⁺ ions were precipitated to be Fe₃O₄ particles inside bacterial cellulose. Then the silver particles were synthesized into the magnetic particle-incorporated bacterial cellulose pellicle by immersing the as-prepared sample in aqueous solution containing of AgNo₃ and glucose. After the silver ion-saturated sample was treated with ammonia gas, the silver particles were formed inside the magnetic particle-incorporated bacterial cellulose. The obtained bacterial cellulose pellicles were rinsed with a large amount of distilled water until it was neutral. Finally, the magnetic and silver particle-incorporated bacterial cellulose were freeze dried and kept in a desicator. The formation of magnetic and silver particles inside bacterial cellulose matrix was investigated by scanning electron microscopy (SEM), X-ray diffraction (XRD), and energy dispersive X-ray (EDX). The percentage loading of each particle in bacterial cellulose matrix were determined by thermogravimetric analysis (TGA). The magnetic field responsive behaviors of the as-prepared samples were studied by vibrating sample magnetometry (VSM) where as the electric field responsive behaviors of the as-prepared samples were studied by two point probe electrometer. Finally, the magnetically and electrically responsive properties of the as-prepared samples were investigated by monitoring the defection of the as-prepared sample in the presence of an applied magnetic and electric fields.