The effect of epigallocatechin-3-gallate (EGCG) on the push out bond strength of ah plus sealer to root dentin

Abstract

Introduction: This study investigated the effect of epigallocatechin-3-gallate (EGCG) from green tea extract used as an endodontic irrigant on the push out bond strength of AH plus sealer to root dentin. Methods: 70 premolars with single root canals were decoronated and instrumented to size F3 (ProTaper Universal) and irrigated with 1 ml of 2.5% NaOCl between each instrument. The roots were divided into 4 groups (n=16) according to the final irrigant: group 1-17% EDTA, group 2-17% EDTA followed by 2.5% NaOCl , group 3-17% EDTA followed by EGCG, and group 4-EGCG. Six additional root canals received 5 ml of 2.5% NaOCl as the final irrigant as a control group. One root from each group (n=1) was prepared for scanning electron microscope evaluation. Then, the root canals were obturated with gutta-percha and AH plus sealer. The root canals were horizontally sectioned into one-millimeter thick slices, and the push out test was performed on root slices. The push out bond strength was analyzed using one-way analysis of variance and Tukey post hoc test. Results: Final irrigation with 17% EDTA followed by EGCG had the highest bond strength (p<.05). The EGCG group demonstrated significantly higher bond strength compared with the EDTA group (p<.05). However, there was no significant difference in bond strength between EDTA with NaOCl and EDTA groups (p>.05). Conclusions: The use of EGCG significantly increased the push-out bond strength between AH plus sealer and root dentin.