Pacific white shrimp (Litopenaeus vannamei) and black tiger prawn (Penaeus monodon) have been significant key species in Thailand agro industry. Monodon Slow Growth Syndrome (MSGS) and Early Mortality Syndrome (EMS) epidemics have been causing tremendous damage to the industry.There were reports on the co-infection of Enterocytozoon hepatopenaei, a microsporidian with these outbreaks in shrimp but no report on other species co-existing in shrimp pond.This research aims to study the infection of E. hepatopenaei in other aquatic macrofauna co-existing in shrimp ponds by molecular technique. The study was conducted in 4 shrimp farms in Chachoengsao province 2 farms and Chanthaburi province 2 farms. Samples of shrimp, aquatic macrofauna, soil and water were collected on the beginning and harvest of the crops.The results indicated that E. hepatopenaei was evident 5 species of aquatic macrofauna : giant freshwater prawn (Macrobrachium rosenbergii), thai vinegar crab (Episesarma mederi), violet vinegar crab (E. versicolor),rice-field crab (Sayamia bangkokensis) and green tidal crab (Varuna litterata).Histopathological results showed the evidence of E. hepatopenaei in hepatopancreatic lumen.In summary, the E. hepatopenaei could be infected not only in shrimp, but also, in other macrofauna living in the same ponds. These could possibly be passage for infection by vectors.This is the first report on the infection of E.hepatopenaei in aquatic macrofauna. .