In this study, adsorption efficiency of 17-alpha methyl testosterone (MT) in single solute and mixed solute with dissolved organic carbon (DOC) by various adsorbents, graphene oxide (GO), magnetic particle (SP), modified GO by attached GO with magnetic particle (GO-SP), silica coated magnetic particle (SiO2-SP), magnetic particle with amino substituted modified silica (NH2-SiO2-SP) and graphene oxide modified magnetic particle with amino grafted silica (GO-NH2-SiO2-SP). The results indicated that the adsorption of MT on GO and GO-NH2-SiO2-SP can describe by pseudo 2 order but GO-SP can described by pseudo 1 order. These three adsorbents can adsorbed MT rapidly in first 10 minutes. SP cannot adsorb MT. Adsorption isotherm of GO, GO-SP and GO-NH2-SiO2-SP was well described by Langmuir isotherm. In mixed solute, the coexisting DOC can enhance the adsorption capacity of MT by multilayer adsorption between DOC and MT. Adsorption of MT in low concentration (µg/L) found that GO that attached on GO-SP and GO-NH2-SiO2-SP had higher MT adsorption capacity than free GO. This might be caused by the agglomeration of free GO sheet. In magnetic separation. It was found that GO attached on GO-SP can be released after ultrasonic. However, GO attached on GO-NH2-SiO2-SP was not released after ultrasonic. The grafted amino group on GO-NH2-SiO2-SP might enhance the strength of bonding between GO and NH2-SiO2-SP.