Phenanthrene is classified as a priority PAH pollutant which is normally found in petroleum contaminated soil. To remediate phenanthrene-contaminated soil, the immobilization of bacteria that has ability for phenanthrene degradation was investigated. This study aims to produce immobilized Pseudomonas sp. J801 for phenanthrene removal from contaminated soil and storage the immobilized bacteria in dried condition. Coconut husk is an agricultural-waste was used as immobilized material which could also absorb PAHs. The study found that 8% (w/v) of coconut husk mixed with cell suspension for 12 hours achieved the bacterial attachment on immobilized material about 1.59×109 CFU/g coconut husk. Moreover, the immobilized bacteria could remove phenanthrene over 74.36% from liquid semi-continuous experiment that added 200 ppm of phenanthrene every 3 days for 24 days. Polymerase Chain Reaction-Denaturing gradient gel electrophoresis also showed that Pseudomonas sp. J801 existed after the immobilized material was used for 24 days; however, free cell did not exist since day 15 of the experiment. Then, it revealed that immobilized bacteria could tolerate to the toxicity of phenanthrene more than free cell. The 150 ppm of phenanthrene-contaminated soil was remediated with 5% (w/v) immobilized bacteria compared to 5% (w/v) immobilized material for 28 days. The result found that the remaining phenanthrene concentrations in the experiments of immobilized bacteria and immobilization material were 2.33% and 6.98%, respectively. However, the phenanthrene removal was clearly differentiate in day 14 that the phenanthrene remaining concentrations in the experiment of immobilized bacteria and immobilized material were 15.23% and 73.09%, respectively. These could be concluded that the immobilized bacteria could improve the efficiency for phenanthrene removal. The ready-to-use immobilized bacteria was also performed by air-drying technique for bacteria storage at room temperature. Preparation of Pseudomonas sp. J801 in 25% (v/v) Luria-Bertani added with 0.5 M sodium chloride then suspended the bacterial cell in 5% (w/v) sucrose phosphate buffer could improve bacteria alive at least 7 days. The immobilized bacteria after storage at room temperature for 14 days showed the efficiency to remove phenanthrene in liquid cultivation about 74.19%. In conclusion, the immobilized bacteria before and after storage showed the phenanthrene removal efficacy indicating that immobilized bacteria have potential to be applied for remediation of phenanthrene-contaminated soil and can be further developed as ready to use bacteria.