Cyclodextrin grafted with chitosan (CD-g-CS) is a substance that has potential applications in many areas. It combines the key propertie of cyclodextrin, a carier property, and the key properties of chitosan, a mucoadhesive property and an antimicrobial property, together. This compound has excellent properties for use in drugs delivery system for a mucosal organ but the application of it is limited because of its poor solubility in water. Quaternized cyclodextrin grafted with chitosan (QCD-g-CS) was synthesized for improving the water solubility. However, the synthesis was only in bench scale. In this research the quaternized cyclodextrin grafted with chitosan (QCD-g-CS) was synthesized for improving the solubility. The quaternized chitosan (QCS) and the QCD-g-CS were prepared by reacting chitosan or the CD-g-CS with glycidyltrimethyl ammonium chloride (GTMAC). The effects of mole ratio, reaction time, and reaction temperature on degree of quaternization (DQ) were investigated. The results indicated the suitable conditions for this reaction is at the mole ratio of 7, the reaction time of 6 hrs, and the temperature of 50 ◦C. The DQ of QCD-g-CS and QCS were found the same as each conditions. For 2 L and 10 L reactor, standard tank reactors were used. Effect of agitation speed was study. It was found that the agitation speed at 500 rpm and 358 rpm is suitable condition for 2 L and 10 L reactor, respectively. For scale-up, this process can be scale-up by choose an equal heat transfer coefficient as a scale-up objective.