LIPOIC ACID-CONTAINING PORPHYRINIC DERIVATIVES FOR MITOCHONDRIA TARGETING APPLICATIONS

Abstract

In this research, an asymmetric water-soluble porphyrin having pyridinium and lipoic acid-based meso-substituents was successfully synthesized. All new compounds were characterized by NMR spectroscopy and mass spectrophotometry. Their optical properties were also investigated by UV-visible and fluorescence spectrophotometry. For the biological studies, low cytotoxicity (IC50 = 25–150 µg/mL) of these compounds in HaCaT and HDFa cells was observed. However, based on ROS generation assay, these compounds did not exhibit antioxidative activity in both kinds of cells. Moreover, mitochondria-targeting evaluation of the synthesized compounds showed that these compounds can be accumulated in the mitochondria of the HaCaT cells.