BIOACTIVE COMPOUNDS FROM ROOTS AND LEAVES OF Clerodendrum inerme

Abstract

This study has evaluated bioactive compounds from roots and leaves of Clerodendrum inerme. CH2Cl2 extracts of plant materials were separated with various chromatography techniques. There were obtained 15 isolates compounds (8 isolates from leaves and 7 isolated from roots), (3β, 22E 24S)-stigmasta-5,22,25-trien-3-ol (1), pectolinarigenin (2), acacetin (3), 5,8-dihydroxy-7,4'-dimethoxyflavone (4), scutellarein-4'-methyl ether (5), ladanein (6), 5-hydroxy-4′,7-dimethoxyflavone (7) and (3β,22E,24S)-stigmasterol-5,22,25-triene-3-yl-glucopyranoside (8) including isolated compounds from roots, stigmasterol (9), n-hexadecyl propionate (10), lupeol laurate (11), β-amyrin decosanoate (12), betulinic acid (13), 6,11,12,16-tetrahydroxy-5,8,11,13-abitetetraen-7-one (14) and 3-O-acetyl-betulinic acid (15). Selected compounds were evaluated biological activity (antimicrobial activity, anti-cholinesterase activity and anti-Aβ disaggregation activity). It was found bioactive compounds from CH2Cl2 extract of C. inerme. Among selected compounds, compound 1 has more active against S. aureus growth, compound 7 has more active against P. aeruginosa growth and compound 3 has more active against C. albicans. Compound 3 has more active against Aβ-aggregation. Furthermore, compound 7 has more active on AChE activity and compound 2 has more active on BChE activity. This study supports that C. inerme is possible source of bioactive compounds especially antimicrobial activity, anti-cholinesterase activity and anti-Aβ digaggregation activity. The structure relation activity of phytochemicals from this study depended on type of its skeleton and the substituents were also observed.