PRODUCTION DEVELOPMENT OF VIOLACEIN FROM Pseudoalteromonas luteoviolacea AND PRODIGIOSIN FROM Pseudoalteromonas rubra USING ADSORBENT RESINS

Abstract

Violacein is a bacterial violet pigment produced by several species of bacteria that currently important for pharmaceutical and cosmatics applications. The other pigment, prodigiosin is red pigment alkaloid comprising conjugated three pyrrole residues. It is produced by several strains of bacteria, particularly genus Serratia. Prodigiosin and other related alkaloids exhibit a broad range of biological activities. The problem of fermentation process for violacein and prodigiosin production in large scale were low productivity and the use of large amount of organic solvent for extraction and isolation process. This research, to use adsorbent resin for the recovery and purification of violacein and prodigiosin from fermentation broth of Pseudoalteromonas luteoviolacea and Pseudoalteromonas rubra, respectively. Of five different adsorbent resins, namely HP20, XAD4, XAD7, XAD16 and XAD1180 were evaluated for efficiency to enhance violacein and prodigiosin production. Dispersion of XAD1180 enhanced the highest yield, particularly when 2% w.v-1 XAD1180 was added to medium after 16 h of cultivation. This method could produce 2-fold increment in violacein production when compared with conventional fermentation and maximum yield of violacein was up to 3.3 g.L-1. And It was found that P. rubra grew well at pH and temperature ranges of 7.6-8.6 and 4-30 °C, respectively. In addition, the regenerated XAD 1180 could be reused additional three times without significant drop in violacein yield. Furthermore research for Production development of prodigiosin using adsorbent resins, we have developed a practical and easy approach that could be applied to improve prodigiosin production. We demonstrated that addition of 1 %w/v of Diaion HP20 could increase total prodigiosin up to 1.6 folds, compared to control. Prodigiosin was highest (8.58 g/L) produced in the culture broth containing 1 %w/v of Diaion HP20. However, the increased amount of resin did not significantly alter production yield but gradual declines in total pigment were observed. And It was found that P. rubra grew well at pH and temperature ranges of 6.0-7.6 and 28-30 °C, respectively. HP20 used in the experiment are expected to be regenerated and retain its adsorption capacity. HP20 was also reused up to four times without a significant drop in the product yield.