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Title: Enhancing passive transport of micro/nano particles into cells by oxidized carbon black
Authors: Kittima Amornwachirabodee
Supaporn Khramchantuk
Prompong Pienpinijtham
Nipan Israsena
Tanapat Palaga
Supason Wanichwecharungruang
Email: No information provided
No information provided
Other author: Chulalongkorn University. Faculty of Science
Chulalongkorn University. Faculty of Medicine
Issue Date: 25-Jun-2018
Publisher: American Chemical Society
Citation: ACS Omega. 3,6 (2018) : p.6833-6840
Abstract: Uses of micro-/nano-sized particles to deliver biologically active entities into cells are common for medical therapeutics and prophylactics and also for cellular experiments. Enhancing cellular uptake and avoiding destruction by lysosomes are desirable for general particulate drug delivery systems. Here, we show that the relatively nontoxic, negatively charged oxidized carbon black particles (OCBs) can enhance cellular penetration of micro- and nano-particles. Experiments with retinal-grafted chitosan particles (PRPs) with hydrodynamic sizes of 1200 ± 51.5, 540 ± 29.0, and 430 ± 11.0 nm (three-sized model particles) indicate that only the sub-micron-sized particles can penetrate the first layer of multilayered liposomes. However, in the presence of OCBs, the micron-sized PRPs and the two submicron-sized PRPs can rapidly enter the interiors of all layers of the multilayered liposomes. Very low cellular uptakes of micro- and submicron-sized PRPs into keratinocytes cells are usually observed. However, in the presence of OCBs, faster and higher cellular uptakes of all of the three-sized PRPs are clearly noticed. Intracellular traffic monitoring of PRP uptake into HepG2 cells in the presence of OCBs revealed that the PRPs did not co-localize with endosomes, suggesting a nonendocytic uptake process. This demonstration of OCB’s ability to enhance cellular uptake of micro- and submicron-particles should open up an easy strategy to effectively send various carriers into cells.
ISSN: 2470-1343
metadata.dc.identifier.DOI: 10.1021/acsomega.8b00487
Type: Article
Appears in Collections:Chula Scholars - 2018

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