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Title: Wax-assisted one-step enzyme-linked immunosorbent assay on lateral flow test devices
Authors: Ishii, Masanori
Pattarachaya Preechakasedkit
Yamada, Kentaro
Orawan Chailapakul
Suzuki, Koji
Citterio, Daniel
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Other author: Chulalongkorn University. Faculty of Science
Issue Date: 10-Jan-2018
Publisher: Japan Society for Analytical Chemistry
Citation: Analytical Sciences. Vol.34, Issue 1 (Jan 10, 2018), p.51-56
Abstract: Lateral flow tests (LFTs) are widely used analytical tools characterized by portability, operator simplicity and short analysis times. A remaining challenge is their limited analytical sensitivity, which in classical immunoassay formats is overcome by enzyme-linked immunosorbent assay (ELISA) formats. The implementation of ELISA to an LFT format however, is hampered by the complexity of the procedure requiring the enzyme substrate addition after sample addition. In this work, a simple method for automation of this procedure without user interference is presented. Originally used sample pads of LFTs have been replaced by hydrophobic wax-modified filter paper-based sample pads to realize a delayed flow a pre-deposited colorimetric ELISA substrate without other alterations to the classical lateral-flow immunoassay format. The performance of the system has been characterized by visualizing flow behavior and final proof-of-concept is provided by a model mouse IgG assay, achieving a limit of detection of 15.8 ng mL−1 from just a single application of the sample solution.
ISSN: 0910-6340 (Print)
1348-2246 (Online)
metadata.dc.identifier.DOI: 10.2116/analsci.34.51
Type: Article
Appears in Collections:Chula Scholars - 2018

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