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Title: Comparison of Four Human Papillomavirus Genotyping Methods : Next-generation Sequencing, INNO-LiPA, Electrochemical DNA Chip, and Nested-PCR
Authors: Pornjarim Nilyanimit
Jira Chansaenroj
Witthaya Poomipak
Kesmanee Praianantathavorn
Sunchai Payungporn
Yong Poovorawan
Email: No information provided
No information provided
Other author: Chulalongkorn University. Faculty of Medicine
Issue Date: Mar-2018
Publisher: Seoul National University, Institute for Cognitive Science
Citation: Annals of Laboratory Medicine. Vol.38, No. 2 (Mar 2018), p.139-146
Abstract: Background : Human papillomavirus (HPV) infection causes cervical cancer, thus necessitating early detection by screening. Rapid and accurate HPV genotyping is crucial both for the assessment of patients with HPV infection and for surveillance studies. Methods : Fifty-eight cervicovaginal samples were tested for HPV genotypes using four methods in parallel: nested-PCR followed by conventional sequencing, INNO-LiPA, electrochemical DNA chip, and next-generation sequencing (NGS). Results : Seven HPV genotypes (16, 18, 31, 33, 45, 56, and 58) were identified by all four methods. Nineteen HPV genotypes were detected by NGS, but not by nested-PCR, INNO-LiPA, or electrochemical DNA chip. Conclusions : Although NGS is relatively expensive and complex, it may serve as a sensitive HPV genotyping method. Because of its highly sensitive detection of multiple HPV genotypes, NGS may serve as an alternative for diagnostic HPV genotyping in certain situations.
ISSN: 2234-3806 (Print)
2234-3814 (Online)
metadata.dc.identifier.DOI: 10.3343/alm.2018.38.2.139
Type: Article
Appears in Collections:Chula Scholars - 2018

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