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Title: Modulated expression of the HIV-1 2LTR zinc finger efficiently interferes with the HIV integration process
Authors: Sutpirat Moonmuang
Somphot Saoin
Koollawat Chupradit
Supachai Sakkhachornphop
Nipan Israsena
Ruttachuk Rungsiwiwut
Chatchai Tayapiwatana
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Other author: Chulalongkorn University. Faculty of Medicine
Issue Date: Oct-2018
Publisher: Portland Press Ltd
Citation: Bioscience Reports. Vol.38, Issue 5 (Oct, 2018), 13 pages
Abstract: Lentiviral vectors have emerged as the most efficient system to stably transfer and insert genes into cells. By adding a tetracycline (Tet)-inducible promoter, transgene expression delivered by a lentiviral vector can be expressed whenever needed and halted when necessary. Here we have constructed a doxycycline (Dox)-inducible lentiviral vector which efficiently introduces a designed zinc finger protein, 2-long terminal repeat zinc-finger protein (2LTRZFP), into hematopoietic cell lines and evaluated its expression in pluripotent stem cells. As a result this lentiviral inducible system can regulate 2LTRZFP expression in the SupT1 T-cell line and in pluripotent stem cells. Using this vector, no basal expression was detected in the T-cell line and its induction was achieved with low Dox concentrations. Remarkably, the intracellular regulatory expression of 2LTRZFP significantly inhibited HIV-1 integration and replication in HIV-inoculated SupT1 cells. This approach could provide a potential tool for gene therapy applications, which efficiently control and reduce the side effect of therapeutic genes expression.
ISSN: 0144-8463 (Print)
1573-4935 (Online)
Type: Article
Appears in Collections:Chula Scholars - 2018

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