Bioactive Compounds From Songkhla Province Mangrove-Derived Endophytic Fungi And From Heart-Leaved Moonseed Tinospora Baenzigeri Forman

Abstract

This study has evaluated biological activities of isolated compounds from Tinospora baenzigeri stems collecting from two different areas, and from mangrove-derived endophytic fungi isolated from trees inhabiting in Songkhla province. Four new rearranged clerodane-type diterpenes (66-69), one new glycoside (70), and six known compounds (61, 71-75), were extracted from plant obtained from local market in Bangkok. Twelve compounds including a new rearranged clerodane-type diterpene (76), a new rearranged clerodane glycoside (77), five known compounds (44, 78-81), and five compounds counting 61, 66, 70 and 74-75 were obtained from plant collected in Bueng Kan Province. Structures of new compounds 66-69 and 76 were confirmed by single-crystal X-ray crystallography technique. Of the 18 compounds, 13 compounds were evaluated for biological activities against four cancer cell lines including Hep-G2, KATO-3, MCF-7 and CaSki ; anti-inflammatory on J774.A1; and α-glucosidase inhibitory activity. Tinobaenzin C (68) exhibited selectivity cytotoxic activity against KATO-3 cell lines with IC50 values of 27.08 µM, while other compounds did not affect to any of the tested cell lines. Only N-trans-feruloyltyramine (74) displayed the weak activity against α-glucosidase with an IC50 value of 0.34 mM for sucrase inhibition and 0.36 mM for maltase inhibition. However, all of the tested compounds were inactive on anti-inflammatory activities. In addition, EtOAc crude extracts from a marine-derived endophytic fungus, Setosphaeria rostrata, cultured in SDB medium were purified and resulted in five new isocoumarin derivatives (84-88), one xanthone (82), and one known isocoumarin (83). The compounds 83 and 85 were subjected to biological activities. Unfortunately, all of them showed to be inactive.