Preparation and protein adsorption of polyion complex thin film of chitosan and chitosan derivatives

Abstract

Charged derivatives of chitosan, N-sulfofurfuryl chitosan (SFC) and N-[(2- hydroxyl-3-trimethylammonium)propyl]chitosan chloride (HTACC), were prepared by reductive alkylation using 5-formyl-2-furansulfonic acid, sodium salt (FFSA) as a reagent and ring opening of glycidyltrimethylammonium chloride (GTMAC) by amino groups of chitosan, respectively. Chemical structures of the charged derivatives were verified by 'H NMR and FTIR analyses. Multilayer thin film of chitosan and its charged derivatives was fabricated by alternate layer-by-layer adsorption on plasma-treated poly(ethylene terephthalate) (treated PET) substrate. Assembly process was monitored by quartz crystal microbalance (QCM). Stratification of multilayer film was demonstrated by water contact angle data. Bioactivity of deposited multilayer film on treated PET substrate was tested against selected proteins having distinctive size and charge. This research promptly suggests that both SFC and HTACC are potential candidates for biomedical application