Coumarins from the roots of Feroniella lucida and their antiplatelet activities

Abstract

In phytochemical investigation of coumarins from Thai medicinal plant in the family Rutaceae, dichloromethane crude extract from the roots of Fe roniella lucid a was selected for separation, purification, structural elucidation and evaluation for anti-platelet aggregation. The chromatographic of dichloromethane crude extract led to the isolation of three new furanocoumarins, feroniellin A (7), feroniellin B (11) and feroniellin c (9), along with eight known furanocoumarins , anisolactone (1), 2", 3"-epoxyanisolactone (2) psoralen (3), bergapten (4), isopimpinellin (5), marmesin (6), oxypeucedanin hydrate (8), and 2", 3"-dihydroxyanisolactone (10). The structures of all isolated compounds were established on the basis of spectroscopic data and compared to literatures. All isolated furanocoumarins were further evaluated for anti-platelet aggregation. The results showed that feroniellin B was only one compound among the isolated furanocoumarins that inhibited ADP-induced platelet aggregation. Importantly, feroniellin B (IC 50 = 0.287 raM) was thirty-nine times more potent than ibuprofen (IC 50 = 11.2 mM), positive control.