Protective study of immunoglobulins prepared from whole cell and capsular antigens of pasteurella multocida

Abstract

The two types of vaccines prepared from Pasteurella multocida that caused fowl cholera were formalin killed whole – cell vaccine and capsular polysaccharide vaccine. Separation of the capsular antigen from saline extracts of P. multocide cultured on tryptose agar was achieved by fractional precipitation from aqueous solution by addition of polar organic solvents. Two groups of three rabbits each were immunized with each type of vaccines. In mouse passive protection test, anti - whole cell globulin and anti – capsular polysaccharide globulin were given subcutaneously to each group of 25 mice, and were challenged intraperitoneally 24 hr later, with living culture at 30 LD₅₀° This investigation was found that whole cell and capsular antigens produced a high immune response in rabbits. The anti – whole cell globulin gave higher passive protection in mice than anti – capsular polysaccharide globuoin.