Bioactive compounds from the stems and stem bark of Feroniella lucida

Abstract

In phytochemical investigation for bioactive compounds from Thai medicinal plants in the family Rutaceae, dichlormethane crude extract from the stems of Feroniella lucida was selected for isolation, purification and structure elucidation. The chromatographic separation of dichloromethane crude extract led to the isolation of a novel furanocoumarin, feroniellin A (7), along with eight known coumarins, psoralen (1), bergapten (2), isopimpinellin (3), anisolactone (4), 2',3'-epoxyanisolactone (5), marmesin (6), feroniellin B (9), and feroniellin C (8). On the other hand, the dried stem bark of this plant was macerated in dichloromethane. This extract was isolated to afford seven triterpenoids. They were three new triterpenoids: feroniellide C (13), feroniellide D (15), and feroniellide E (16) and four known triterpenoids; N-methyl anthranilic ester triterpenoid (10-12) and isovaleric acid ester triterpenoid (14). The structures of all isolated compounds were elucidated by spectroscopic methods as well as comparison with previous literature data. All of the isolated compounds were tested for inhibitory activity of acetylcholinesterase and cytotoxicity on HeLa and KB cell lines. Compounds 3, 6, 7, 8, 9, 11, and 12 showed inhibitory activity of acetylcholinesterase with MIC value of 0.500 mg/mL. In addition, compound 14 exhibited significant cytotoxicity activity against HeLa cell line with IC[subscript 50] value of 8.2 [micro]g/mL, while compounds 10, 15 and 16 also showed potent cytotoxicity against KB cell line with IC[subscript 50] value of 5.2, 4.1 and 3.4 [micro]g/mL, respectively.