Studies on the incidence of helicobacter pylori infections by polymerase chain reactions and the relationship of clinical signs of H. pylori infections in canine stomachs

Abstract

A study on helicobacter pylori infection in canine stomach was performed. A total of 75 biopsied samples of cardia, fundus, body and pyloric antrum from necropsied dogs, submitted to the Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, during April, 2003 to June, 2004, were investigated. The objective of this study was focused on the prevalence of H. pylori in canine stomach by polymerase chain reaction (PCR) in comparison to histochemistry and immunohistochemistry (IHC) as well as relationship between clinical significance of and gastric H. pylori infection. The histopathological results revealed 60.0% (45/75) as mild gastritis 64.44% (29/45), moderated gastritis 11.11% (5/45) and severe gastric 24.44% (11/45) and no histopathological lesions as 40.0% (30/75). The presence of helicobacter spp. using hematoxylin & eosin (H&E) staining, showed positive results at 17.33% (13/75), warthin starry staining (WSS), 46.67% (35/75) and the helicobacter spp. was localized in the luminal crypt 18.67% (14/75), gastric pit 22.67% (17/75), the gastric gland 21.33% (16/75), and the gastric epithelium 8% (6/75), by IHC with rabbit polyclonal anti-H. pylori antibody 30.67% (23/75) and by PCR 10.67% (8/75), but none of H. pylori could be detected in all samples. There was no significantly different in histopathological changes in any part of the stomach (p>0.05). The H. pylori diagnosis by polymerase chain reaction (PCR) in comparison to WSS and IHC was not significantly different (p>0.05). There were no relationship between pathological study by H&E, WSS and IHC, especially PCR and clinical signs of H. pylori infections in canine stomachs (p>0.05). The present study revealed significantly different correlations for Helicobacter spp. detection between H&E and WSS (p<0.001)