Cloning and expression of pick gene from streptomyces venezuelae in streptomyces species

Abstract

PicK, a cytochrome P450 monooxygenase encoded by picK gene of Streptomyces venezuelae , biocatalyzes the hydroxylation of narbomycin to picromycin. The objectives of the research are to clone picK in Streptomyce s species and Escherichai coli. The recombinant picK could be expressed as protein detected at molecular weight of 45,000 daltons (SDS-PAGE) and the PicK cytochrome P450 monooxygenase activity could be determined by carbon monoxide (CO) difference spectra analysis. On the other hand, no sign of the picK expression and its P450 activity could be observed by SDS-PAGE and CO-difference spectra analyses in Streptomyces species. The results seem to imply that lac promoter is unsuitable for gene expression in Streptomyces.