Cyclic amp phosphodiesterase inhibitory activity of stem bank of Croton oblongifolius Roxb. from amphoe Tha-Uthen, Nakhon Phanom province

Abstract

The stem bark of Croton oblongifolius Roxb. from Nakhonphanom province was extracted with hexane and ethyl acetate. The crude extracts were separated using column chromatography and the structures of isolated components were elucidated using spectroscopic techniques. The crude extract gave ledol (1), 5-hydroxy-3,7,4'-trimethoxyflavone (2) and 5-hydroxy-3,7,3',4'-tetramethoxyflavone. (3), Pterodoltriol D (4) and campesteryl-3-O-beta-D-glucopyranoside, stigmasteryl-3-O-beta-D-glucopyranoside, and beta-sitosteryl-3-O-beta-D-glucopyranoside (5). Each compound was tested for inhibitory activity on cyclic AMP phosphodiesterase following the bioassay using malachite green method. The results showed that the compound 1, 2 and 3 showed high inhibitory activity.