Effects of biliary obstruction and indomethacin on renal functions in rats

Abstract

Study the effects of biliary obstruction and indomethacin on renal functions in rats. Rats were divided into five groups, sham control, sham control with vehicle, sham control with indomethacin, biliary obstruction without indomethacin and biliary obstruction with indomethacin groups. Rats were subjected to bile duct ligation for five days. Five days after operation, rats were anesthetized and measured systemic hemodynamics, renal hemodynamics and renal function with and without the intravenous administration of 5 mg/kg BW indomethacin, an inhibitor of prostaglandin production. In biliary obstructed rats, the effective renal blood flow (ERBF) rose by 62.6% and glomerular filtration rate (GFR) by 52.5% (p<0.05), although systemic hemodynamics and renal functions were not changed in comparing with the other groups. Administration of indomethacin to the five day biliary obstructed rats, the ERBF and GFR declined to be equal with the sham control group, whereas systemic hemodynamics and renal function were not different from the other groups. These data demonstrated that, in rats with biliary obstruction by bile duct ligation, renal hemodynamics is increased, and the enhanced synthesis of vasodilatory prostaglandins which are blocked by indomethacin in this study may be raise renal hemodynamics.