Formation and biocompatibility of multilayer films assemblemd from charged derivatives of chitosan

Abstract

Charged derivatives of chitosan, N-[(2-hydroxyl-3-trimethylammonium)propyl] chitosan chloride (HTACC), N-succinyl chitosan (SCC), and N-sulfofurfuryl chitosan (SFC) were prepared by ring opening of glycidyltrimethylammonium chloride (GTMAC), ring opening of succinic anhydride (SA) by amino groups of chitosan, and reductive alkylation using 5-formyl-2-furansulfonic acid, sodium salt (FFSA) as a reagent, respectively. The chemical structures of the charged derivatives were verified by 1H NMR and FTIR. Multilayer films of charged derivatives of chitosan and selected oppositely charged polyelectrolytes on the surface-treated poly(ethylene terephthalate) (treated PET) substrates were fabricated by alternate layer-by-layer assembly. The assembly process was monitored by quartz crystal microbalance (QCM). Stratification of the multilayer film was demonstrated by water contact angle data. The coverage of the assembled films was characterized by atomic force microscopy and ATR-FTIR spectroscopy. Biological responses of the assembled films as assessed by in vitro cell adhesion and proliferation of fibroblasts showed slight alternating trend depending on the outermost layer. The results also suggest that these three charged derivatives of chitosan are potential candidates for biomedical applications.