Evaluation of gold nanoparticles effect on cellular senescence change in preadipocyte (3T3-L1) and fibroblast (NIH 3T3) cell lines induced with hydrogen peroxide

Abstract

Currently, gold nanoparticles (AuNPs) are popular to the implementation for many fields because of their exclusive features and high stability which are notably different from other nanoparticles. Indeed, skin care and anti-aging product use AuNPs for delivering active ingredients to the skin. Although many studies reported the benefits of AuNPs in terms of anti- senescent cells, there were some studies claimed that they also induced senescent cells. This study aimed to evaluate the effect of AuNPs on the cellular senescence change in preadipocyte and fibroblast cells. There were the uses of hydrogen peroxide for inducing senescent cells and the different size and concentration of AuNPs for this experiment. To test the effect of AuNPs to cytotoxicity on cells, aging phenotypes, free radical on cells, and cellular senescence mechanism. The results of this study were found that 50 ppm concentration of AuNPs size in 10 nm had cytotoxicity on both types of cells. AuNPs size in 10 nm and 20 nm were able to protect the occurrence of senescent cells phenotype notably on the preadipocyte cells only. On the contrary, AuNPs size in 10 nm and 20 nm were not able to stimulate the occurrence of free radical in both types of cells at all. From this, it was concluded that the cells response on aging was independent to free radical on cells. Furthermore, AuNPs size in 10 nm and 20 nm enabled to increase the SIRTUIN1 and p53 mRNA expressions, but decrease the C/EBPa and PPARg mRNA expressions on the preadipocyte cells only.