Genetic variation of Green Peafowls Pavo muticus Linnaeus, 1766 in Northern and Western Thailand based on Microsatellite DNA

Abstract

Genetic variation of Green Peafowl (Pavo muticus) between northern and western part of Thailand (northern population is from Ping basin and western population is from Huai Kha Khaeng basin) were analyzed by using 8 microsatellite loci of 25 individual per site. Genomic DNA was extracted from feather tip and was amplified by polymerase chain reaction (PCR). Amplification with 8 microsatellite primers of HUJ002, LEI166, MCW034, MCW069, MCW080, MCW068, MCW295, and MCW330, the results showed that the total allelic numbers per locus were 2, 2, 4, 2, 3, 2, 4, and 2, respectively. Mean number of alleles per locus of Green Peafowl from northern and western part of Thailand were 2.13 and 2.25, respectively. The mean of expected heterozygosity (HE) of Green Peafowl population from northern part and western part were 0.40 and 0.42, respectively and it was not significantly different between populations. Green Peafowl from northern part and western part were small and large population, respectively and had random mating because from Hardy-Weinberg assumption, Green Peafowl from northern part and western part at all loci no deviated from this assumption. Genetic differentiation within both populations of Green Peafowl was higher than genetic differentiation between populations. The genetic distance of Green Peafowl between northern and western populations were 0.1956.