Bioactive compounds from the heartwood of dalairo Anaxagorea luzonensis A. Gray

Abstract

The chemical constituent investigation from the heartwood of Anaxagorea luzonensis A. Gray disclosed seventeen compounds. By means of physical properties and spectroscopic evidences, the structures of seventeen compounds could be elucidated as 1,3,5-trihydroxy-4-(3-hydroxy-3-metylbutyl)xanthone (1), orobol (2), naringenin (3), aromadendrin (4), keampferol (5), quercetin (6), taxifolin (7), 1,3,6-trihydroxy-5-methoxyxanthone (8), 1,3,5-trihydroxy-4-prenylxanthone (9), 1,3,5,6-tetrahydroxyxanthone (10), daidzein (11), biochanin A (12), genistein (13), 3,6-dihydroxy-1,5-dimethoxyxanthone (14), 8-prenylnaringenin (15), stigmasterol (16) and 6-deoxy-isojacareubin (17). Among of them, compounds (3), (4), (11), (13), (16) and (17) have been first reported as chemical constituents in A. luzonensis. The results of bioactivity test revealed that compounds (1) and (2) had strong activity with IC50 2.36, and 2.57 M against acetylcholinesterase and 2.25, 3.66 M against butyrylcholinesterase, respectively. Additionally, compounds (6) and (9) revealed strong activity with IC50 1.27 and 4.90 M against butyrylcholinesterase, respectively. Compound (6) exhibited the highest scavenging activity toward DPPH radical with IC50 0.09 mM and decolorizing ABTS with TEAC value 2.88. Xanthones (1), (8), (9) and (10) exhibited high inhibitory activity against phosphodiesterase comparing with other flavonoids.