Comparison and prediction of retention of disubstituted aromatic compounds in capillary electrokinetic chromatography

Abstract

Retention index (I), rather than retention factor (k) was found to be a more reasonable parameter for comparison of the relative affinity of disubstituted benzenes in MEEKC and MEKC, due to independent of I with the SDS surfactant concentration. MEKC and MEEKC may give similar or different I values, depending on types of moieties. With known I and Kow for alkylbenzenes as references in MEKC and MEEKC, the values of log Kow for disubstituted benzenes can be estimated from the observed I values, where Kow is the octanol-water distribution constant. In addition, a group additive approach can be used to predict I for disubstituted benzenes with different moieties from the average observed I for the disubstituted benzenes with the same moieties. However, electronic effects and/or intramolecular interaction may result in the different observed I from prediction.