Combination of gold nanoparticles and lycorine for enhancing anti-inflammatory activity

Abstract

Inflammation is a complex biological response of tissues to harmful stimuli, such as pathogens. NSAIDs are commonly used to reduce inflammatory activities; however, some types of NSAIDs have side-effects on patients. Importantly, in this study attention the naturally occurring substances from plant to development of new drugs to treat inflammatory activities. Recently, Lycorine is one of the major alkaloids that have been isolated from the Amaryllidaceae plant has been reportedly possessed the anti-inflammatory activity. It is a good therapeutic candidate against inflammation which can inhibit the expression of cytokines.

Recently, gold nanoparticles (AuNPs) have been used in medicinal applications due to their unique properties such as high chemical stability, high biocompatility. AuNPs as carriers for the delivery of drugs, genetic materials and proteins resulting in enhanced their activities. For the anti-inflammation, AuNPs also inhibit the expression of NF-kappa B, a protein that acts to stimulate inflammation. In order to enhance the activation of the Lycorine, AuNPs have been employed.

Finally, this work compared the performance of lycorine, Au@P, and Ly+Au@P towards RAW 264.7 cells using LPS-induced NO tests. The results demonstrated that Lycorine could inhibit similar to budesonide in NO production. The inhibition doses was 10 µM for Ly+Au@P This work showed a successful inhibition agent derived from the excellent combination of Lycorine and Au@P. The synthesis of AuNPs using pullulan that has a particles size about 10 nm. The shape is spherical shape by TEM technigue.