The Effect of dengue and chikungunya virus mixed infection in Aedes albopictus (C6/36) cells line and Aedes spp. mosquitoes

Abstract

Aedes aegypti and Aedes albopictus are an important mosquito’s vector disease that supported dengue and chikungunya virus transmission in Asia and South East Asia countries. In recently, Ae. albopictus has a trend to displace to Ae. aegypti habitat in Africa, Europe and America, and Ae. albopictus is a great mainly vectors which contribute to mutant chikungunya virus transmission than Ae. aegypti, A226V substitute on E1 gene. All 4 serotype of dengue viruses are widely spread in Thailand annually which presents cocirculation in the same region. Additionally, reemergence of chikungunya outbreaks are reported in southern parts of Thailand in 2009, more than 24,029 cases of chikungunya fever have been reported. Although dual infections of two viruses in sera’s patient was reported but mixed infection in mosquito vector have been rarely studied. To evaluate whether both virus can be transmitted to human host by passing through single mosquito. We used Aedes albopictus C6/36 cell line and female Aedes spp. mosquitoes as a model of viral transmission in vitro and in vivo, respectively. The results show mixed-infection in C6/36 cell line and viral production are increase. Competitive suppression was found in two mosquitoes with using high viral concentration or equal titer and surprisingly, Ae. aegypti are supposed to carrying two viruses particle in single mosquitoes whereas Ae. albopictus presents only one strain of viral mixed-infection. The results provided the new knowledge in order to understand the effect of vector-virus interactions and virus transmission which might be applies to predict the outbreak and control the diseases in future.