Assay of mebendazole by using the reaction of carbonyl group and 2, 4-dinitrophenylhydrazine

Abstract

The reaction of 2,4-dinitrophenylhydrazine (2,4-DNPH) and ketone yields an orange precipitate of 2,4-dinitrophenylhydrazone (2,4-DNPH’ zone) derivative which is insoluble in water, and it was developed in order to assay mebendazole by visible-spectrometric method. The 2,4-DNPH' zone derivative obtained was identified by thin layer chromatography, ultraviolet-visible spectrometry, infrared spectrometry, H¹ -nuclear magnetic resonance spectrometry and mass spectrometry. The various conditions affecting the reaction were examined. It was shown that mebendazole in tablets could be assayed with a good result by 2,4-DNPH method comparable to official method that prescribed in USP XXI.