Evaluation of cosmetic activities of the extracts from mushrooms of the family boletaceae In Thailand

Abstract

Mushroom of the family Boletaceae (Boletes mushroom) is one of edible mushrooms and can be found in Thailand. There are many studies reporting that it had high content of polyphenols and showed good antioxidant activities when compared with other mushrooms. However, there are still few studies about cosmetic activities of Boletes mushroom extracts especially Boletes mushrooms in Thailand. The present study aimed to evaluate the content of bioactive compounds of fruiting body extracts and culture broth extracts from Boletes mushrooms locally grown in Thailand including phenolic compounds, flavonoid compounds, carbohydrate compounds and protein compounds. Moreover, in vitro cosmetic activities including free radical scavenging activities (DPPH and ABTS assays), anti-tyrosinase, anti-collagenase and anti-elastase activities of these extracts were evaluated. The relationship between their cosmetic activities and bioactive compound contents of mushroom extracts was examined with Pearson’s correlation test. The results showed that Boletes mushroom extracts from fruiting body had higher content of bioactive compounds than culture broth extracts except carbohydrate compound. In addition, they also showed higher cosmetic activities than culture broth extracts. TISTR 55 FB extract showed the highest phenolic (113.17 ± 1.17 milligram gallic acid equivalent per gram extract) and flavonoid contents (12.52 ± 1.35 milligram quercetin equivalent per gram extract) and the greatest DPPH (IC50 13.62 ± 0.70 microgram/millilter) and ABTS (IC50 23.58 ± 0.30 microgram/milliliter) free radical scavenging activities. Moreover, TISTR 37 FB extract showed potent anti-collagenase (63.81 ± 4.74%) and anti-elastase activities (44.44 ± 2.25%) at the concentration of 500 microgram/milliliter when compared with other mushroom extracts studied. In addition, no or low correlation between their bioactive compounds and cosmetic activities was observed except DPPH scavenging activity which showed good correlation with phenolic content of fruiting body extracts. The results indicated that type and part of mushroom extracts affected their bioactive compounds and cosmetic activities. The present study exhibited that the mushroom extracts of the family Boletaceae in Thailand were promising for further development as new active natural ingredients in cosmetic products.