Biodegradation of iprodione by a bacterium isolated from soil

Abstract

Iprodione is a dicarboximide fungicide that is widely used to control fungi and target pests in wineyard and golf courses. Iprodione is naturally transformed to its main intermediate with relatively higher toxicity, 3,5-dichloroaniline (3,5-DCA). Since bioremediation is considered to be an effective method to reduce remaining toxic compounds, the bacterial screening and isolation were carried out to obtain the bacterial isolate able to degrade both chemicals. Through the enrichment technique, Pseudomonas aeruginosa strain I4 was isolated. It was identified based on the basic of comparison of 16S rDNA sequence analysis. The strain could degrade 40 µM iprodione and 200 µM 3,5-DCA up to 73.99 % and 98.29 %, respectively, within 24 hours. Among various co-substrates, 5 mM succinic acid could enhance both iprodione and 3,5-DCA degradation. Furthermore, catechol 1,2-dioxygenase (C12DO) of strain P. aeruginosa strain I4, respectively representing the ortho-cleavage pathway Moreover, the result showed that strain I4 could degraded other dicarboximide fungicide and other chloroaniline.