Bioactive compounds from the stems of Cudrania cochinchinensis

Abstract

The investigation for bioactive compounds from the stems of Cudrania cochinchinensis Lour, led to the isolation of one new compound, 8,2',3'-trihydroxy- 7,6'-dimethoxyisoflavan-4-ol (5) along with six known compounds, 5,7-dihydroxy-4-methoxyisoflavone or biochanin A (1), 7,3'-dihydroxy-2',4'-dimethoxyisoflavanone or violanone (2), 5,7-dihydroxyflavanone or pinnocembrin (3), 7-hydroxy-2',4'-dimethoxy isoflavanone or sativanone (4), 7-hydroxy-4'-methoxyflavone (6), cycloartenone (7) from hexane and dichloromethane crude extracts. The structure of 8,2',3'-trihydroxy-7,6'-dimethoxyisoflavan-4-ol, new flavonoid, was elucidated by spectroscopic methods. In addition, compound 2 and 5 showed significant free radicals scavenging activity on DPPH (IC50 138.8 µM and 61.2 µM), while compound 1, 3 and 4 were found to be high in-vitro cytotoxicity against KB cell lines. Compound 5 also showed highest %NBT reduction inhibition (29.60) among the isolated compounds from this plant and its degree of inhibition activity was closed to allopurinol (34.65) which was used as an oral drug for treating gout.