Chitosan-magnetite nanoparticles via "Click" chemistry

Abstract

Chitosan-magnetite nanoparticles are proposed under Cu(I) free “click” chemistry. Chitosan is modified with propargyl bromide to obtain chitosan derivative whereas magnetite nanoparticles are coupled with silane followed by treating with sodium azide to obtain magnetite nanoparticles surface modified with azide group. Both chitosan with alkyne group derivative and magnetite nanoparticles surface modified with azide group are conjugated without Cu(I) catalyst in mild condition under “click” chemistry. The chitosan-mangnetite nanoparticles show hydrodynamic radius approximately 200 nm and have a good disperibility in buffer solution with various pHs. A preliminary study on DNA separation using the chitosan-magnetite nanoparticles via “click” chemistry confirms a similar level as chitosan-magnetite via non “click” chemistry in extracting DNA of E.coli.