Formulation and evaluation of Facial-Patch containing Asiaticoside using polysaccharide gel from fruit-hulls of Durian

Abstract

Asiaticoside was extracted from Centella asitica known as Gotu Kola in Umbelliferae family. It has been used firstly as the medicinal plant in India. Asiaticoside has a various applications, enhances the wound healing and widely uses in skin illness. Asiaticoside is intermediate polar substance, low water solubility. It can dissolve in propylene glycol, the solubility ratio of asiaticoside in propylene glycol is 1: 9.5. The tween 20 increased the solubility of asiaticoside 1.5 times by weight. In this study, polysaccharide gel (PG) extracted from dried fruit-hulls of durian was used to formulate the facial-patch and was developed as bilayer film containing asiaticoside in adhesive layer and backing layer. Casting preparation of the facial-patch composed of 5 % PG as a film forming agent and 5 % ethylcellulose as a backing layer. The plasticizers of adhesive layer were 15 % w/w sorbitol and 4.75 % propylene glycol which were used for solubilizing of asiaticoside. This facial-patch provided the most satisfactory mechanical properties such as tensile strength, %elongation, work of failure, and Young’s modulus including appearance. In the diffusion studied, 90 % asiaticoside was diffused from the PG facial-patch within 1 hour. The irritation studied in 6 normal skin rabbits, none of them showed irritation, but irritation was happened in the 6 abraded skin rabbits. In the prophetic patch test using 200 volunteers, the 2 of them showed slightly erythema. Clinical test of moisturizing and antiwrinkle effect were performed in 20 volunteers using DermaLab® moisture probe instrument to measure transepidermal water loss (TEWL) and elasticity on the forehead area and macroscopic photograph was taking to evaluate the wrinkle lines. The TEWL and Young’s modulus were significantly decreased (p<0.05) resulted in the improvement of elasticity of the skin after 4 weeks of application, in addition reducing of wrinkle on forehead. Macroscopic photograph was also support this result.