Sutureless human amniotic membrane fixation using bubaline fibrin glue for corneal surface reconstruction in experimental rabbits

Abstract

This study was designed to compare the efficacy of the buffalo-blood fibrin glue or bubaline fibrin glue with the suture material for the fixation of the human amniotic membrane on the corneal surface in experimental rabbits. Bubaline blood was drawn aseptically and processed by cryoprecipitation to extract fibrinogen and thrombin. Ten of rabbits were divided into 2 groups, the control (n=3) and the treatment (n=7). The membrane was fixed with Nylon and the bubaline fibrin glue in the control and the treatment groups respectively. The surgical time was recorded. Animals were reared for 14 days and collected the duration of the presence of fluorescein staining, tear production, corneal clarity score, pain score and clinical outcomes. The animals were euthanized and the eyes were enucleated. The eyes were histopathologically processed for wounding study. The surgical time and corneal clarity score in the treatment group was shorter than the control group significantly (P<0.05). Epithelialization pattern in the treatment group was more organized than that in the control group, and the membrane was attached more closely than the control group. Use of the bubaline fibrin glue could provide better clinical outcomes and less surgical time than the suture materials.