Effect of extraction solvents on biological activities of Murraya Paniculata leaves in cosmetic application

Abstract

The objective of this study was to evaluate and compare the antioxidant activity and tyrosinase inhibition activity of Murraya paniculata (Linn.) Jack leaves extract (MPE) obtained from different extraction solvents for the use of cosmetic purposes. The result showed that 75 % ethanolic extract presented the highest antioxidant activity (IC50 = 1.43 mg/mL), the total phenolic content of 66.12 mg GAE/g and the total flavonoids content of 7.83 mg QE/g. All of the obtained extracts had low tyrosinase inhibitory activity. The final MPE lotion contained 1 %w/w of 75 % ethanolic extract dissolved in 50 %w/w of propylene glycol, 20 %w/w of glycerin, and 7.5 %w/w of ethanol. Regarding stability study, the MPE lotion was stored at 4 °C and 40 °C for 1 month. Total flavonoid content remaining in the lotion and antioxidant activity of the MPE lotion were monitored during the storage. The results at both storage temperatures showed that no significant decrease of the total flavonoid content and antioxidant activity were observed from the 1 % MPE lotion. This meant that this MPE lotion had high potential to be developed for cosmetic purpose.