Gold nanoparticles in CLP model and mechanism in bone marrows derived macrophages cell for sepsis severity attenuation

Abstract

Sepsis is still a major worldwide- public health problem as a leading cause of the high mortality rate condition. Sepsis is the inflammatory condition in host due to bacterial infections. Sepsis severity depends on the individual's immune responses and the organism virulence factor. The current strategies for sepsis treatment are based, mainly on, symptomatic treatment with antibiotics. However, the adjuvant therapy in sepsis is interesting. Gold nanoparticles are anti-inflammatory drug from the ancient time which is still currently used for the treatment of rheumatoid arthritis. Thus, we hypothesized that gold nanoparticles could be effective in reducing an inflammation in sepsis and testes in bone marrow (BM)-derived macrophages (in vitro) and cecal ligation and puncture (CLP) mouse model (in vivo). After treat with gold nanoparticles, the pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) was reduced in both in vitro and in vivo. But, the anti-inflammation cytokine (IL-10) was increased in vitro but decreased in vivo. However, CLP mice showed the higher survival rate with gold nanoparticles treatment. the bactericidal property and the M2 macrophages induction property of gold nanoparticles are also demonstrated. In conclusion, gold nanoparticles attenuated sepsis through the immune modulation and bactericidal activity. Further studies on the mechanisms and the translation to patients are interesting.