Isolation of α-glucosidase inhibitors and cytotoxic compounds from heartwood of mansonia gagei drumm

Abstract

Phytochemical and biological investigations were carried out on the heartwoods of M. gagei. Twelve known compounds (1–11, 29), ten new sesquiterpenoids (12–21), seven novel compounds (22–28) and one new neolignan (30) were isolated. Their structures were elucidated by spectroscopic data analysis (UV-Vis, FTIR, 1D, 2D NMR and HRESIMS), and their absolute configurations were established by calculation of ECD data. Isolated compounds were evaluated for antioxidant, cytotoxic activity and enzyme inhibitory activity against α-glucosidase. Interestingly, mansonialactam (21) showed significant inhibitory activity against α-glucosidase from the yeast with the IC50 value of 0.2 ± 0.05 mM which was 468 times lower than that of acarbose (93.63 ± 0.49). Kinetic study revealed that 21 and 24 were uncompetitive inhibitors. In terms of antioxidant activity, 11, 25 and 27 exhibited very good activities with the IC50 values of 13.66 ± 0.42, 12.76 ± 0.15 and 13.19 ± 0.72 µM, respectively. In regard to cytotoxic activity, 18 possessed the highest cytotoxicity on lung cancer cell line A549 with an IC50 value of 15.59 ± 2.49 µM.