Qualitative and quantitative analyses of fatty acid and steroid in sandworms Perinereis sp.

Abstract

The percentage of fat of Perinereis nuntia farm-raised sandworm aged 8 months (20.96±0.73%) was higher than those of aged 2, 4 and 6 months (17.35±0.08%), while that of P. nuntia wild-caught sandworm was 13.43±2.83%. The most abundant fatty acids of the farm-raised sandworm were C16:0, C18:1 n-9 and C18:2 n-6 while those of the wild-caught were C16:0, C18:1 n-7 and C18:0. The ratio of SFA : MUFA : PUFA of all of the farm-raised was 1.3 : 1.0 : 1.0 while the ratio of those of the wild-caught in summer and winter season were 4.0 : 2.2 : 1.0 and 2.3 : 1.5 : 1.0, respectively. The ratio of ARA : EPA : DHA and n-3 : n-6 fatty acid of the farm-raised sandworm were 1.6 : 1.3 : 1.0 and 1.0 : 3.0, to stand in aged 4, 6 and 8 months, whereas ratio of those of the wild-caught were different. Total fatty acid in age 6 months of P. aibuhitensis was higher than that of P. nuntia and P. quatrefagesi. The ratio of SFA : MUFA : PUFA and n-3 : n-6 fatty acid of P. aibuhitensis and P. quatrefagesi of farm-raised were similar to those of P. nuntia. The major component of the lipid was phospholipids, especially phosphatidylcholine (PC). Cholesterol of P. nuntia farm-raised sandworm was (60 mg/ 100g ww) less than that of the wild-caught (81 mg/ 100g ww), while in difference species was slightly different.